Anti-Plakophilin-2; clone 8H6

Analysis Note

ControlA431 cell lysate

Application

Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in A431 and UM-SCC-38 cells. (Sobolik-Delmaire, T., et al. (2010). Journal of Investigative Dermatology. 130:2638–2646.)

Anti-Plakophilin-2 Antibody, clone 8H6 detects level of Plakophilin-2 & has been published & validated for use in Western Blotting, ICC.

Research CategoryCell Structure

Research Sub CategoryAdhesion (CAMs)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Plakophilin-2 (PKP2) is a member of the Armadillo/β-catenin family. It contains eight ARM repeats and can exist in two slice isoforms (2a and 2b). Plakophilin-2 is highly abundant in simple and stratified epithelial cells; but is also expressed in non-epithelial cells, such as the myocardium and lymph node follicles. In epithelial cells, it is localized to desmosomal plaques and plays an important role in cell-cell adhesion processes. Plakophilin-2 also maintains the cell-cell junctions of cardiac tissue, and is essential for proper cardiac function. Mutations in the plakophilin-2 gene cause arrhythmogenic right ventricular cardiomyopathy 9, a condition characterized by partial breakdown of the myocardium of the right ventricle, electrical instability, and sudden death. Plakophilin-2 has also been implicated in the development of cancer.

Immunogen

MBP-tagged recombinant protein corresponding to human Plakophilin-2.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blot in A431 cell lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected Plakophilin-2 in 10 µg of A431 cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~97 kDa observed